|
KMID : 0371620020170010011
|
|
Journal of Wonkwang Medical Science
2002 Volume.17 No. 1 p.11 ~ p.20
|
|
|
Change of Mechanical Properties and MHC Protein in the Soleus and Medial Gastrocnemius Muscles of Hindlimb Suspended Rats
|
|
Lee Jae-Hyo
Lee Moon-Young
Lee Sung-Ho
|
|
|
Abstract
|
|
|
|
The present study was designed to evaluate the changes of mechanical properties of contraction and myosin heavy chain (MHC) protein in the soleus and medial gastrocnemius muscles following hindlimb suspension in rats. After 14 days of hindlimb suspension, contraction time, half relaxation time, maximum twitch tension, maximum tetanic tension and fatigue index were measured in the soleus and medial gastrocnemius muscles, also MHC was measured in those muscles by means of immunohistochemical study.Body weight and muscle weight were decreased significantly in hindlimb suspended rats. In hindlimb suspended rats, mechanical properties including contraction time, half relaxation time, maximum twitch tension and maximum tetanic tension were decreased significantly and muscle fatigue was increased, which was more prominent in the soleus muscle than the gastrocnemius muscle. MHC type Ⅰ was decreased significantly and MHC type Ⅱx which was not observed in control was increased in the soleus muscle following hindlimb suspension, which represented that the slow muscle fibers converted to the fast muscles fibers. In the medial gastrocnemius muscle following hindlimb suspension, MHC type Ⅰ and type Ⅱx were decreased significantly and MHC type Ⅱb was increased significantly. These results suggest that MHC of the slow muscle fibers converted to MHC of the fast muscle fibers during hindlimb suspension, which was corresponded to changes of mechanical properties of contraction.
|
|
|
KEYWORD
|
|
|
myosin heavy chain immunohistochemical study hindlimb suspended rats
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|